A Simple and Rapid Spectrophotometric Method for Nitrite Detection in Small Sample Volumes

A simple, rapid, and environmentally-friendly spectrophotometric method for nitrite detection was developed. Detection based on a redox reaction with iodide ions in an acidic condition. The evaluated by detecting the increase absorbance of colored product iodine at 362 nm wavelength. To obtain good performance, concentration, hydrochloric acid time were optimized. In optimal condition, developed provided linear range 0.0625 to 4.00 mg L−1 (r = 0.9985), 10 min, limit 25 µg L−1, quantitation 85 L−1. This showed repeatability (RSD < 9.21%), high sample throughput (9 samples min−1), accuracy (recovery 88 ± 2 99.5 0.4%). has potential be used crime scene investigations as rapid screening test gunshot residue via detection.